Xanthine Oxidase Inhibitor, Allopurinol, Prevented Oxidative Stress, Fibrosis, and Myocardial Damage in Isoproterenol Induced Aged Rats

Figure 1

Hematoxylin and Eosin staining showed inflammatory cell infiltration and necrosis in left ventricle of heart section of rats treated with ISO. Control rats showed normal architecture with very well-shaped cardiomyocytes in left ventricle of heart (a). ISO treatment increased necrosis to cardiomyocytes and the mononuclear inflammatory cells infiltration in left ventricle of heart (b) which was normalized by allopurinol treatment (c). Moreover control rats showed normal baseline collagen around cardiomyocytes (a) which was significantly increased in ISO treated rats (b). Allopurinol treatment further prevented the fibrosis in heart of ISO treated rats. Sirius red staining showed fibrosis (red color) in left ventricle of heart section of rats treated with ISO. (a, d) Control; (b, e) ISO; and (c, f) ISO + allopurinol, magnification 40x.

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