Xanthine Oxidase Inhibitor, Allopurinol, Prevented Oxidative Stress, Fibrosis, and Myocardial Damage in Isoproterenol Induced Aged Rats

Table 2

Effect of allopurinol on biochemical parameter in plasma, heart, and kidney of isoprenaline treated rats.

Parameters	Groups
	Control	ISO	ISO + allo.
Plasma
AST (U/L)	27.56 ± 3.22^a	55.13 ± 2.11^b	40.20 ± 4.80^a
ALT (U/L)	29.29 ± 3.45^a	49.96 ± 6.33^a	35.89 ± 6.06^a
ALP (U/L)	58.28 ± 5.92^a	80.35 ± 5.58^ab	54.89 ± 6.12^ac
MDA (nmol/mL)	38.77 ± 1.84^a	64.41 ± 4.89^b	41.72 ± 1.68^a
NO (nmol/mL)	3.87 ± 0.27^a	6.80 ± 0.65^b	5.44 ± 0.54^a
APOP (nmol/mL equivalent to chloramine-T)	228 ± 10.68	656.90 ± 101.18	390.63 ± 61.52
Catalase (U/min/mg protein)	20.83 ± 3.52	14.17 ± 2.01	41.67 ± 6.79
GSH (ng/mg protein)	11.48 ± 0.52^a	7.55 ± 0.40^b	13.33 ± 1.39^c
CK-MB (U/L)	38.89 ± 7.78^a	120.56 ± 15.26^b	62.22 ± 7.78^a
Heart
NO (nmol/mL)	14.12 ± 0.91^a	15.87 ± 2.23^a	14.45 ± 0.65^a
MDA (nmol/mL)	36.08 ± 0.81^a	48.51 ± 2.41^b	45.00 ± 1.00^b
APOP (nmol/mL equivalent to chloramine-T)	632.06 ± 33.84	706.67 ± 44.95	655.87 ± 24.60
Catalase (U/min/mg protein)	154.17 ± 17.77	68.33 ± 8.33	231.67 ± 25.35
Kidney
NO (nmol/mL)	22.59 ± 1.66^a	29.19 ± 3.11^a	23.51 ± 2.32^a
MDA (nmol/mL)	39.03 ± 1.04^a	53.26 ± 2.08^b	39.00 ± 0.00^a
APOP (nmol/mL equivalent to chloramine-T)	687.62 ± 23.80^a	786.83 ± 122.40^a	727.30 ± 48.75^a
Catalase (U/min/mg protein)	38.33 ± 4.22	17.50 ± 1.12	40.83 ± 4.73

Values are presented as mean ± SEM. N = 5–7 in each group or otherwise specified. One-way ANOVA with Bonferroni tests were done as post hoc test. Values are considered significant at . a versus b: control versus ISO; b versus c: ISO versus allopurinol treatment.