miR-103 Regulates Oxidative Stress by Targeting the BCL2/Adenovirus E1B 19 kDa Interacting Protein 3 in HUVECs

Figure 4

H₂O₂ upregulated the expression of BNIP3 in HUVECs. (a) BNIP3 protein expression in HUVECs cultured in 200 μM H₂O₂ for different time periods (left). Quantification of BNIP3 by band densitometry analysis (right). (b) BNIP3 protein expression in HUVECs cultured with H₂O₂ at the indicated concentration for 6 h (left). Quantification of BNIP3 by band densitometry analysis (right). (c) Western blot analysis of BNIP3 protein expression in HUVECs pretreated with or without salidroside and induced by H₂O₂. (d) Western blot analysis of BNIP3 protein expression in HUVECs stably expressing miR-103 treated with H₂O₂. GAPDH was used as the loading control. ; .

(a)

(b)

(c)

(d)