Research Article

Optimisation of an Advanced Oxidation Protein Products Assay: Its Application to Studies of Oxidative Stress in Diabetes Mellitus

Figure 1

Improved reproducibility of the modified AOPPTIOC assay after centrifugation (5800 g, 5 min) of the microplate and transfer of supernatant. Four random plasma samples were taken and optical density was measured at a wavelength of 340 nm following (a) the original protocol and (c) our modified protocol. Optical density was also measured at 595 nm in (b) the original protocol and (d) our modified protocol in order to examine sample turbidity. Bars represent triplicate optical density values obtained, with each type of shading representing a different plasma sample. Numbers under the appropriate bars represent the % coefficient of variation (% CV) of the triplicate measurements for that particular sample.