Optimisation of an Advanced Oxidation Protein Products Assay: Its Application to Studies of Oxidative Stress in Diabetes Mellitus
Table 2
Evaluation of the linearity of AOPPTIOC measurements.
Plasma sample number
Dilution (%)
Mean blank
Mean test
AOPPTIOC (M chloramine T equivalents)
OD340
OD340
AOPPTIOC1a
x dilution
AOPPTIOC2b
x dilution
1
5
0.068
0.032
4.37
87.4
2.09
41.8
10
0.123
0.131
17.17
171.7
4.14
41.4
20
0.209
0.602
78.38
391.9
8.85
44.3
2
5
0.075
0.040
5.35
107.1
2.31
46.3
10
0.130
0.161
21.07
210.7
4.59
45.9
20
0.224
0.641
83.46
417.3
9.14
45.7
3
5
0.123
0.058
7.76
155.2
2.79
55.7
10
0.220
0.202
26.48
264.8
5.15
51.5
20
0.404
0.800
104.13
520.6
10.20
51.0
OD340: optical density at 340 nm. aAOPPTIOC1: AOPPTIOC concentration calculated from original optical density values using the standard curve. bAOPPTIOC2: square root of AOPPTIOC1.
