Reactive Oxygen Species Are Required for Human Mesenchymal Stem Cells to Initiate Proliferation after the Quiescence Exit

Figure 1

Correlation between the reactive oxygen species (ROS) level and the proliferative status of endometrial mesenchymal stem cells. (a) ROS level and cell fraction in the S-phase of the cell cycle measured by flow cytometry 48 hours after the cell seeding versus seeding density (1 ML = 30 000 cells/cm²); (b) ROS level and cell fraction in the S-phase of the cell cycle versus time interval after the cell seeding at 0.3 ML density; (c) and (d) show ROS level versus percentage of cells in the S-phase in (a) and (b). ML: cell monolayer; : Pearson coefficient for a linear correlation between the ROS level and the S-phase cell fraction; ROS level is expressed in arbitrary units , where is a measured carboxy-H2DCF-DA signal, and is a background autofluorescence signal. All data are presented as mean ± SD ().

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