Intracellular Oxidant Activity, Antioxidant Enzyme Defense System, and Cell Senescence in Fibroblasts with Trisomy 21

Figure 2

ROS and mitochondrial superoxide anion detection by fluorescence microscopy in fibroblasts with trisomy 21 (T21F) () and controls (CF) (). Fibroblasts were grown in 96-well microplates and incubated for one hour with Hank’s buffered salt solution (untreated) or supplemented with 100 µM TBHP. The observed green fluorescent signal corresponds to the oxidation of the CM-H2DCFDA probe in response to intracellular ROS production in (a). The red fluorescent signal corresponds to the oxidation of the MitoSOX probe in response to superoxide radical production in the mitochondria (b). Images were taken with a fluorescence microscope at a total magnification of 200x.

(a)

(b)