Intracellular Oxidant Activity, Antioxidant Enzyme Defense System, and Cell Senescence in Fibroblasts with Trisomy 21

Figure 7

Effect of cell passages on relative mRNA expression of SOD1, SOD2, GPx, and catalase (CAT) in fibroblasts with trisomy 21 (T21F) () and controls (CF) (). mRNA expression was analyzed by quantitative real-time PCR in fibroblasts at low and high passages. Expression data were normalized by the means of the double delta Ct method. Results at low (a) and high passages (b) are expressed as mean ± SEM. Statistical differences were analyzed by Student’s -test: ; .

(a)

(b)