Intracellular Oxidant Activity, Antioxidant Enzyme Defense System, and Cell Senescence in Fibroblasts with Trisomy 21

Figure 8

Effect of cell passages on p53 and p21 expression in fibroblasts with trisomy 21 (T21F) () and controls (CF) (). (a) Representative Western blot of p53 and p21 in cell lysates in M-PER. (b) p53 and p21 expression was calculated from optical densities of the bands, measured by an imaging technique and normalized by β-actin. Results were normalized to the band intensities measured in untreated fibroblasts at low passages and expressed as mean ± SEM. (c) p53 and p21 were also quantified by ELISA in cell lysates of T21F and CF. Results were normalized to the optical densities measured in untreated fibroblasts at low passages and expressed as mean ± SEM. Statistical differences were analyzed by Student’s -test: T21F versus CF () and high versus low passages (; ).

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