Table of Contents Author Guidelines Submit a Manuscript
Oxidative Medicine and Cellular Longevity
Volume 2015 (2015), Article ID 509241, 17 pages
http://dx.doi.org/10.1155/2015/509241
Research Article

Intracellular Oxidant Activity, Antioxidant Enzyme Defense System, and Cell Senescence in Fibroblasts with Trisomy 21

1Center for Biomedical Network Research on Rare Diseases (CIBERER), 08028 Barcelona, Spain
2Biochemistry and Molecular Biology Research Center for Nanomedicine, Vall d’Hebron Research Institute, 08035 Barcelona, Spain
3Maternal and Child Health and Development Network II (SAMID II), Institute of Health Carlos III, 28029 Madrid, Spain
4Paediatric Endocrine Service, Vall d’Hebron University Hospital, 08035 Barcelona, Spain

Received 18 December 2014; Accepted 19 February 2015

Academic Editor: Ersin Fadillioglu

Copyright © 2015 Víctor Rodríguez-Sureda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. Mégarbané, A. Ravel, C. Mircher et al., “The 50th anniversary of the discovery of trisomy 21: the past, present, and future of research and treatment of Down syndrome,” Genetics in Medicine, vol. 11, no. 9, pp. 611–616, 2009. View at Publisher · View at Google Scholar · View at Scopus
  2. D. L. Nelson and R. A. Gibbs, “The critical region in trisomy 21,” Science, vol. 306, no. 5696, pp. 619–621, 2004. View at Publisher · View at Google Scholar · View at Scopus
  3. H. E. Lockstone, L. W. Harris, J. E. Swatton, M. T. Wayland, A. J. Holland, and S. Bahn, “Gene expression profiling in the adult Down syndrome brain,” Genomics, vol. 90, no. 6, pp. 647–660, 2007. View at Publisher · View at Google Scholar · View at Scopus
  4. M. S. Cheon, M. Dierssen, S. H. Kim, and G. Lubec, “Protein expression of BACE1, BACE2 and APP in down syndrome brains,” Amino Acids, vol. 35, no. 2, pp. 339–343, 2008. View at Publisher · View at Google Scholar · View at Scopus
  5. A. M. W. Coppus, D. Fekkes, W. M. A. Verhoeven, S. Tuinier, and C. M. van Duijn, “Plasma levels of nitric oxide related amino acids in demented subjects with Down syndrome are related to neopterin concentrations,” Amino Acids, vol. 38, no. 3, pp. 923–928, 2010. View at Publisher · View at Google Scholar · View at Scopus
  6. P. M. Sinet, “Metabolism of oxygen derivatives in Down's syndrome,” Annals of the New York Academy of Sciences, vol. 396, pp. 83–94, 1982. View at Publisher · View at Google Scholar · View at Scopus
  7. B. W. Brooksbank and R. Balazs, “Superoxide dismutase, glutathione peroxidase and lipoperoxidation in Down's syndrome fetal brain,” Brain Research, vol. 318, no. 1, pp. 37–44, 1984. View at Google Scholar · View at Scopus
  8. F. V. Pallardó, P. Degan, M. d'ischia et al., “Multiple evidence for an early age pro-oxidant state in Down syndrome patients,” Biogerontology, vol. 7, no. 4, pp. 211–220, 2006. View at Publisher · View at Google Scholar · View at Scopus
  9. J. Muchová, M. Sustrová, I. Garaiová et al., “Influence of age on activities of antioxidant enzymes and lipid peroxidation products in erythrocytes and neutrophils of down syndrome patients,” Free Radical Biology and Medicine, vol. 31, no. 4, pp. 499–508, 2001. View at Publisher · View at Google Scholar · View at Scopus
  10. H. D. Teixeira, R. I. Schumacher, and R. Meneghini, “Lower intracellular hydrogen peroxide levels in cells overexpressing CuZn-superoxide dismutase,” Proceedings of the National Academy of Sciences of the United States of America, vol. 95, no. 14, pp. 7872–7875, 1998. View at Publisher · View at Google Scholar · View at Scopus
  11. S. I. Liochev and I. Fridovich, “The role of O2- in the production of HO: in vitro and in vivo,” Free Radical Biology and Medicine, vol. 16, no. 1, pp. 29–33, 1994. View at Publisher · View at Google Scholar · View at Scopus
  12. C. W. White, K. B. Avraham, P. F. Shanley, and Y. Groner, “Transgenic mice with expression of elevated levels of copper-zinc superoxide dismutase in the lungs are resistant to pulmonary oxygen toxicity,” The Journal of Clinical Investigation, vol. 87, no. 6, pp. 2162–2168, 1991. View at Publisher · View at Google Scholar · View at Scopus
  13. N. Nakao, P. Brundin, K. Funa, O. Lindvall, and P. Odin, “Trophic and protective actions of brain-derived neurotrophic factor on striatal DARPP-32-containing neurons in vitro,” Developmental Brain Research, vol. 90, no. 1-2, pp. 92–101, 1995. View at Publisher · View at Google Scholar · View at Scopus
  14. B. Halliwell and J. M. C. Gutteridge, “The definition and measurement of antioxidants in biological systems,” Free Radical Biology and Medicine, vol. 18, no. 1, pp. 125–126, 1995. View at Publisher · View at Google Scholar · View at Scopus
  15. P. E. Coskun and J. Busciglio, “Oxidative stress and mitochondrial dysfunction in Down's syndrome: relevance to aging and dementia,” Current Gerontology and Geriatrics Research, vol. 2012, Article ID 383170, 7 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  16. Y. Chen, E. McMillan-Ward, J. Kong, S. J. Israels, and S. B. Gibson, “Mitochondrial electron-transport-chain inhibitors of complexes I and II induce autophagic cell death mediated by reactive oxygen species,” Journal of Cell Science, vol. 120, no. 23, pp. 4155–4166, 2007. View at Publisher · View at Google Scholar · View at Scopus
  17. S. Arbuzova, T. Hutchin, and H. Cuckle, “Mitochondrial dysfunction and Down's syndrome,” BioEssays, vol. 24, no. 8, pp. 681–684, 2002. View at Publisher · View at Google Scholar · View at Scopus
  18. S. P. M. Crouch, R. Kozlowski, K. J. Slater, and J. Fletcher, “The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity,” Journal of Immunological Methods, vol. 160, no. 1, pp. 81–88, 1993. View at Publisher · View at Google Scholar · View at Scopus
  19. H. Aebi, “Catalase in vitro,” Methods in Enzymology, vol. 105, pp. 121–126, 1984. View at Publisher · View at Google Scholar
  20. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, “Protein measurement with the Folin phenol reagent,” The Journal of Biological Chemistry, vol. 193, no. 1, pp. 265–275, 1951. View at Google Scholar · View at Scopus
  21. P. K. Smith, R. I. Krohn, G. T. Hermanson et al., “Measurement of protein using bicinchoninic acid,” Analytical Biochemistry, vol. 150, no. 1, pp. 76–85, 1985. View at Publisher · View at Google Scholar · View at Scopus
  22. M. W. Pfaffl, “A new mathematical model for relative quantification in real-time RT-PCR,” Nucleic acids research, vol. 29, no. 9, p. e45, 2001. View at Publisher · View at Google Scholar · View at Scopus
  23. P. Martín-Gallán, A. Carrascosa, M. Gussinyé, and C. Domínguez, “Biomarkers of diabetes-associated oxidative stress and antioxidant status in young diabetic patients with or without subclinical complications,” Free Radical Biology and Medicine, vol. 34, no. 12, pp. 1563–1574, 2003. View at Publisher · View at Google Scholar · View at Scopus
  24. P. Martín-Gallán, A. Carrascosa, M. Gussinye, and C. Domínguez, “Estimation of lipoperoxidative damage and antioxidant status in diabetic children: relationship with individual antioxidants,” Free Radical Research, vol. 39, no. 9, pp. 933–942, 2005. View at Publisher · View at Google Scholar · View at Scopus
  25. G. P. Dimri, X. Lee, G. Basile et al., “A biomarker that identifies senescent human cells in culture and in aging skin in vivo,” Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 20, pp. 9363–9367, 1995. View at Publisher · View at Google Scholar · View at Scopus
  26. M. Lee, D.-H. Hyun, P. Jenner, and B. Halliwell, “Effect of overexpression of wild-type and mutant CU/ZN-superoxide dismutases on oxidative damage and antioxidant defences: relevance to Down's syndrome and familial amyotrophic lateral sclerosis,” Journal of Neurochemistry, vol. 76, no. 4, pp. 957–965, 2001. View at Publisher · View at Google Scholar · View at Scopus
  27. B. Halliwell and M. Whiteman, “Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?” British Journal of Pharmacology, vol. 142, no. 2, pp. 231–255, 2004. View at Publisher · View at Google Scholar · View at Scopus
  28. A. Sevanian and P. Hochstein, “Mechanisms and consequences of lipid peroxidation in biological systems,” Annual Review of Nutrition, vol. 5, pp. 365–390, 1985. View at Publisher · View at Google Scholar · View at Scopus
  29. E. K. Ahmed, C. R. Picot, A.-L. Bulteau, and B. Friguet, “Protein oxidative modifications and replicative senescence of WI-38 human embryonic fibroblasts,” Annals of the New York Academy of Sciences, vol. 1119, no. 1, pp. 88–96, 2007. View at Publisher · View at Google Scholar · View at Scopus
  30. N. Sitte, M. Huber, T. Grune et al., “Proteasome inhibition by lipofuscin/ceroid during postmitotic aging of fibroblasts,” The FASEB Journal, vol. 14, no. 11, pp. 1490–1498, 2000. View at Publisher · View at Google Scholar · View at Scopus
  31. R. E. Pacifici and K. J. A. Davies, “Protein, lipid and DNA repair systems in oxidative stress: the free-radical theory of aging revisited,” Gerontology, vol. 37, no. 1–3, pp. 166–180, 1991. View at Publisher · View at Google Scholar · View at Scopus
  32. J. Kedziora and G. Bartosz, “Down's syndrome: a pathology involving the lack of balance of reactive oxygen species,” Free Radical Biology and Medicine, vol. 4, no. 5, pp. 317–330, 1988. View at Google Scholar · View at Scopus
  33. A. Conti, F. Fabbrini, P. D'Agostino et al., “Altered expression of mitochondrial and extracellular matrix genes in the heart of human fetuses with chromosome 21 trisomy,” BMC Genomics, vol. 8, article 268, 2007. View at Publisher · View at Google Scholar · View at Scopus
  34. M. Perluigi, F. di Domenico, A. Fiorini et al., “Oxidative stress occurs early in Down syndrome pregnancy: a redox proteomics analysis of amniotic fluid,” Proteomics—Clinical Applications, vol. 5, no. 3-4, pp. 167–178, 2011. View at Publisher · View at Google Scholar · View at Scopus
  35. J. Busciglio, J. Busciglio, B. A. Yankner, and B. A. Yankner, “Apoptosis and increased generation of reactive oxygen species in Down's syndrome neurons in vitro,” Nature, vol. 378, no. 6559, pp. 776–779, 1995. View at Publisher · View at Google Scholar · View at Scopus
  36. R. C. Iannello, P. J. Crack, J. B. De Haan, and I. Kola, “Oxidative stress and neural dysfunction in Down syndrome,” Journal of Neural Transmission, Supplement, no. 57, pp. 257–267, 1999. View at Google Scholar · View at Scopus
  37. P. A. Szweda, B. Friguet, and L. I. Szweda, “Proteolysis, free radicals, and aging,” Free Radical Biology and Medicine, vol. 33, no. 1, pp. 29–36, 2002. View at Publisher · View at Google Scholar · View at Scopus
  38. E. Roat, N. Prada, R. Ferraresi et al., “Mitochondrial alterations and tendency to apoptosis in peripheral blood cells from children with Down syndrome,” FEBS Letters, vol. 581, no. 3, pp. 521–525, 2007. View at Publisher · View at Google Scholar · View at Scopus
  39. D. Valenti, G. A. Manente, L. Moro, E. Marra, and R. A. Vacca, “Deficit of complex I activity in human skin fibroblasts with chromosome 21 trisomy and overproduction of reactive oxygen species by mitochondria: involvement of the cAMP/PKA signalling pathway,” Biochemical Journal, vol. 435, no. 3, pp. 679–688, 2011. View at Publisher · View at Google Scholar · View at Scopus
  40. D. Valenti, A. Tullo, M. F. Caratozzolo et al., “Impairment of F1F0-ATPase, adenine nucleotide translocator and adenylate kinase causes mitochondrial energy deficit in human skin fibroblasts with chromosome 21 trisomy,” Biochemical Journal, vol. 431, no. 2, pp. 299–310, 2010. View at Publisher · View at Google Scholar · View at Scopus
  41. M. A. Pritchard and I. Kola, “The ‘gene dosage effect’ hypothesis versus the ‘amplified developmental instability’ hypothesis in Down syndrome,” Journal of Neural Transmission. Supplement, no. 57, pp. 293–303, 1999. View at Google Scholar · View at Scopus
  42. S. E. Antonarakis, R. Lyle, E. T. Dermitzakis, A. Reymond, and S. Deutsch, “Chromosome 21 and Down syndrome: from genomics to pathophysiology,” Nature Reviews Genetics, vol. 5, no. 10, pp. 725–738, 2004. View at Publisher · View at Google Scholar · View at Scopus
  43. A. Gimeno, J. L. García-Giménez, L. Audí et al., “Decreased cell proliferation and higher oxidative stress in fibroblasts from down syndrome fetuses. Preliminary study,” Biochimica et Biophysica Acta, vol. 1842, no. 1, pp. 116–125, 2014. View at Publisher · View at Google Scholar · View at Scopus
  44. J. B. de Haan, F. Cristiano, R. Iannello, C. Bladier, M. J. Kelner, and I. Kola, “Elevation in the ratio of Cu/Zn-superoxide dismutase to glutathione peroxidase activity induces features of cellular senescence and this effect is mediated by hydrogen peroxide,” Human Molecular Genetics, vol. 5, no. 2, pp. 283–292, 1996. View at Publisher · View at Google Scholar · View at Scopus
  45. I. T. Lott, “Antioxidants in Down syndrome,” Biochimica et Biophysica Acta, vol. 1822, no. 5, pp. 657–663, 2012. View at Publisher · View at Google Scholar · View at Scopus
  46. T. R. Garlet, E. B. Parisotto, G. D. S. de Medeiros et al., “Systemic oxidative stress in children and teenagers with Down syndrome,” Life Sciences, vol. 93, no. 16, pp. 558–563, 2013. View at Publisher · View at Google Scholar · View at Scopus
  47. F. L. Muller, M. S. Lustgarten, Y. Jang, A. Richardson, and H. Van Remmen, “Trends in oxidative aging theories,” Free Radical Biology and Medicine, vol. 43, no. 4, pp. 477–503, 2007. View at Publisher · View at Google Scholar · View at Scopus
  48. J. F. Passos, G. Saretzki, S. Ahmed et al., “Mitochondrial dysfunction accounts for the stochastic heterogeneity in telomere-dependent senescence,” PLoS Biology, vol. 5, no. 5, article e110, 2007. View at Publisher · View at Google Scholar · View at Scopus
  49. V. S. Romanov, V. A. Pospelov, and T. V. Pospelova, “Cyclin-dependent kinase inhibitor p21Waf1: contemporary view on its role in senescence and oncogenesis,” Biochemistry, vol. 77, no. 6, pp. 575–584, 2012. View at Publisher · View at Google Scholar · View at Scopus
  50. Y. Y. Sanders, H. Liu, X. Zhang et al., “Histone modifications in senescence-associated resistance to apoptosis by oxidative stress,” Redox Biology, vol. 1, no. 1, pp. 8–16, 2013. View at Publisher · View at Google Scholar · View at Scopus
  51. R. Mirzayans, B. Andrais, A. Scott, and D. Murray, “New insights into p53 signaling and cancer cell response to DNA damage: implications for cancer therapy,” Journal of Biomedicine and Biotechnology, vol. 2012, Article ID 170325, 16 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  52. M. Salemi, R. A. Condorelli, C. Romano et al., “CASP3 protein expression by flow cytometry in Down's syndrome subjects,” Human Cell, vol. 27, no. 1, pp. 43–45, 2014. View at Publisher · View at Google Scholar · View at Scopus
  53. R. Seidl, S. Fang-Kircher, B. Bidmon, N. Cairns, and G. Lubec, “Apoptosis-associated proteins p53 and APO-1/Fas (CD95) in brains of adult patients with Down syndrome,” Neuroscience Letters, vol. 260, no. 1, pp. 9–12, 1999. View at Publisher · View at Google Scholar · View at Scopus
  54. A. Sawa, F. Oyama, N. J. Cairns, N. Amano, and M. Matsushita, “Aberrant expression of bcl-2 gene family in Down's syndrome brains,” Molecular Brain Research, vol. 48, no. 1, pp. 53–59, 1997. View at Publisher · View at Google Scholar · View at Scopus
  55. N. Rueda, J. Flórez, and C. Martínez-Cué, “Apoptosis in Down's syndrome: lessons from studies of human and mouse models,” Apoptosis, vol. 18, no. 2, pp. 121–134, 2013. View at Publisher · View at Google Scholar · View at Scopus
  56. A. Seifert, L. A. Allan, and P. R. Clarke, “DYRK1A phosphorylates caspase 9 at an inhibitory site and is potently inhibited in human cells by harmine,” FEBS Journal, vol. 275, no. 24, pp. 6268–6280, 2008. View at Publisher · View at Google Scholar · View at Scopus
  57. A. Laguna, M.-J. Barallobre, M.-Á. Marchena et al., “Triplication of DYRK1A causes retinal structural and functional alterations in Down syndrome,” Human Molecular Genetics, vol. 22, no. 14, pp. 2775–2784, 2013. View at Publisher · View at Google Scholar · View at Scopus
  58. J.-M. Liao, X. Zhou, Y. Zhang, and H. Lu, “MiR-1246: a new link of the p53 family with cancer and Down syndrome,” Cell Cycle, vol. 11, no. 14, pp. 2624–2630, 2012. View at Publisher · View at Google Scholar · View at Scopus