Adenosine 2B Receptor Activation Reduces Myocardial Reperfusion Injury by Promoting Anti-Inflammatory Macrophages Differentiation via PI3K/Akt Pathway

Figure 6

Confocal staining of p-Akt and immune cells in the heart. Hearts were harvested from mice with 40 min/10 min IR injury for staining to determine the localization of p-Akt expression. (a) In the ischemic area or lower anterior wall of the left ventricle, higher p-Akt expression (red staining) was colocalized with CD45 signal (green staining) in all groups. Scale bar: 20 m. (b) The majority of strong p-Akt expression (red staining) was colocated with CD206 expression (green staining), which is a specific marker of M2 macrophages. Scale bar: 20 m. Arrows indicate coexpression of p-Akt and CD45 or CD206. 4′,6-Diamidino-2-phenylindole (DAPI, blue staining) was used to stain the nuclei. IR: ischemia and reperfusion injury; BAY: BAY 60-6583.

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