Figure 2: EDA attenuates CGNs neurosis induced by IAA. CGNs were preincubated with or without EDA for 2 h followed by exposure to 50 μM IAA for another 4 h. (a) CGNs were stained with FDA and PI (200x magnification). (b) Quantitative analysis of necrotic cells from three representative photomicrographs was represented as a percentage of the total number of cells counted. # versus Ctrl; * and ** versus IAA alone group.