PRMT1 and PRMT4 Regulate Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage in SIRT1-Dependent and SIRT1-Independent Manners

Figure 2

SIRT1 overexpression attenuates oxidative stress-induced RPE cell apoptosis. (a, b) ARPE-19 cells were transfected with empty vector, SIRT1 WT, or SIRT1 H363Y plasmids. After 24 h, the medium was changed to serum-free medium and 250 μM H₂O₂ was added for 24 h. (a) Cell viability was measured by the MTT assay. The data represent the means ± SEM of three independent experiments, each performed in triplicate. versus empty vector, versus empty vector + 250 μM H₂O₂, and versus SIRT1 WT + 250 μM H₂O₂. (b) Cell extracts were subjected to Western blotting with the indicated antibodies. The data represent the means ± SEM of three independent experiments. versus empty vector, versus empty vector + 250 μM H₂O₂, and versus SIRT1 WT + 250 μM H₂O₂ (n.s. = nonspecific).

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