PRMT1 and PRMT4 Regulate Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage in SIRT1-Dependent and SIRT1-Independent Manners

Figure 4

The knockdown of PRMT1 or PRMT4 attenuates oxidative stress-induced RPE cell damage and PRMT1 expression regulates SIRT1 expression. (a) ARPE-19 cells were transfected with scramble, PRMT1, or PRMT4 siRNA according to the reverse transfection method. After 36 h, cell extracts were subjected to Western blotting with the indicated antibodies. (b, c) ARPE-19 cells were transfected with scramble, PRMT1, or PRMT4 siRNA according to the reverse transfection method. After 24 h, the medium was changed to serum-free medium and 250 μM H₂O₂ was added for 24 h. (b) Cell viability was measured by the MTT assay. The data represent the means ± SEM of three independent experiments, each performed in triplicate. versus scramble siRNA, versus scramble siRNA + 250 μM H₂O₂. (c) Cell extracts were subjected to Western blotting with the indicated antibodies. The data represent the means ± SEM of three independent experiments. versus scramble siRNA, versus scramble siRNA + 250 μM H₂O₂ (n.s. = nonspecific).

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