PRMT1 and PRMT4 Regulate Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage in SIRT1-Dependent and SIRT1-Independent Manners

Figure 5

PRMT1 and PRMT4 expression is increased in the RPE layer of streptozotocin-treated rats. Eyeballs were enucleated from vehicle-treated and STZ-treated rats and cryosections were prepared. (a, b) PRMT1 (a) and PRMT4 (b) expressions were measured by immunohistochemistry analysis (C: choroid, RPE: retinal pigment epithelium, PL: photoreceptor layer, OLM: outer limiting membrane, ONL: outer nuclear layer, OPL: outer plexiform layer, INL: inner nuclear layer, IPL: inner plexiform layer, and GCL: ganglion-cell layer). Representative images were from at least three independent experiments. To quantify the DAB signaling, semiautomated analysis protocol was performed as described in Section 2.

(a)

(b)