Protective Role of Nuclear Factor E2-Related Factor 2 against Acute Oxidative Stress-Induced Pancreatic β-Cell Damage
Nrf2 silencing sensitizes MIN6 cells to oxidative stressor-induced cell damage. ((a) and (c)) Concentration-dependent decrease of cell viability in Nrf2-KD and Scr cells caused by H2O2 (a) and glucose oxidase (c) exposure. Cell viability was assessed by MTT assay following a 24 hr treatment. (b) ATP content in Scr and Nrf2-KD cells following a 24 hr H2O2 treatment. ((d) and (e)) Immunoblotting of cleaved Caspase-3 (C-Casp-3), Caspase-3 (Casp-3), and cleaved PARP (C-PARP) in MIN6 cells exposed to H2O2 for indicated time and concentrations. β-Actin was used as a loading control. S, Scr; N, Nrf2-KD. Values in (a), (b), and (c) are means ± SD. . versus Scr with the same treatment.
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