Protective Role of Nuclear Factor E2-Related Factor 2 against Acute Oxidative Stress-Induced Pancreatic β-Cell Damage

Figure 6

Preactivation of NRF2 protects MIN6 cells from H₂O₂-induced cytotoxicity. ((a) and (c)) ARE-luciferase activity measured in MIN6 cells. The cells were challenged with CDDO-Im (a) or DMF (b) for 4 hrs. ((b) and (d)) Effects of CDDO-Im and DMF pretreatment on H₂O₂-induced cytotoxicity. Following a 4 hr pretreatment with CDDO-Im (b) or DMF (d) cells were exposed to H₂O₂ for 12 hrs. Cell viability was assessed by MTT assay. (e) Cells were pretreated with 10 μM tBHQ for 16 hrs followed by a 6 hr H₂O₂ exposure. Apoptosis markers cleaved Caspase-3 (C-Casp-3) and cleaved PARP (C-PARP) were determined using immunoblotting and quantified by ImageJ 1.44. . versus nonpretreated cells with the same treatment.