Polymethoxyflavone Apigenin-Trimethylether Suppresses LPS-Induced Inflammatory Response in Nontransformed Porcine Intestinal Cell Line IPEC-J2

Table 1

Sequence of primer sets used for quantitative real-time.

Gene symbol	Accession number	Primer sequences	Product size (bp)	Efficiency		Reference
IL-8	NM_213867	F 5′-AGAGGTCTGCCTGGACCCCA-3′	126	1.972	0.999	[28]
		R 5′-GGGAGCCACGGAGAATGGGT-3′
IL-6	NM_214399	F 5′-TTCACCTCTCCGGACAAAAC-3′	122	1.970	0.995	[29]
		R 5′-TCTGCCAGTACCTCCTTGCT-3′
TNF-	NM_214022	F 5′-TTCCAGCTGGCCCCTTGAGC-3′	146	1.873	0.982	[30]
		R 5′-GAGGGCATTGGCATACCCAC-3′
COX-2	NM_214321	F 5′-AGAAGCGAGGACCAGCTTTC-3′	215	1.905	0.981	NCBI/Primer-Blast
		R 5′-AAAGCGGAGGTGTTCAGGAG-3′
Hsp70	NM_001123127	F 5′-GCCCTGAATCCGCAGAATA-3′	152	2.0	0.993	[31]
		R 5′-TCCCCACGGTAGGAAACG-3′
CycA	NM_214353	F 5′-GCGTCTCCTTCGAGCTGTT-3′	160	1.907	0.998	[30]
		R 5′-CCATTATGGCGTGTGAAGTC-3′
HPRT	NM_001032376	F 5′-GGACTTGAATCATGTTTGTG-3′	91	1.963	0.997	[32]
		R 5′-CAGATGTTTCCAAACTCAAC-3′