OGG1 Involvement in High Glucose-Mediated Enhancement of Bupivacaine-Induced Oxidative DNA Damage in SH-SY5Y Cells

Figure 4

The effect of high glucose, bupivacaine, or NAC on OGG1 and cleaved caspase-9 protein expression as detected by western blot. Con: SH-SY5Y cells of group control. HG: SH-SY5Y cells were exposed to 50 mM glucose for 8 d. Bup: SH-SY5Y cells were treated with 1.5 mM bupivacaine for 24 h. HG + Bup: SH-SY5Y cells were incubated with 50 mM glucose for 8 d and then treated with 1.5 mM bupivacaine for 24 h. HG + Bup + NAC: cells treated with 50 mM glucose for 8 d and then pretreated with 5 mM NAC for 6 h prior to 1.5 mM bupivacaine exposure for 24 h. (a) The effect of high glucose on OGG1 and cleaved caspase-9 protein expression induced by bupivacaine. (b) The effect of NAC on OGG1 and cleaved caspase-9 protein expression induced by high glucose and bupivacaine. Data are presented as mean ± SD () .

(a)

(b)