Hydrogen Sulfide Donor GYY4137 Protects against Myocardial Fibrosis

Figure 7

Effect of GYY4137 on TGF-β1/Smad2 expression in cardiac fibroblasts. Neonatal rat cardiac fibroblasts were pretreated with different concentrations of GYY4137 for 4 h followed by Ang II (10⁻⁷ M) stimulation for an additional 24 h. Cell lysates were tested for TGF-β1 (a) and Smad2 protein (b) expression by western blotting. GAPDH was probed as a loading control. Plots represent mean ± SEM from 4 independent experiments. Cells treated with culture medium served as vehicle control (con.). Statistical significance: , compared with con. group; , compared with Ang II stimulation alone.

(a)

(b)