Melatonin Attenuates Oxidative Damage Induced by Acrylamide In Vitro and In Vivo

Figure 3

Effect of MT on ACR-induced DNA damage in PC12 cells and in rats. PC12 cells were cultured with vehicle controls, ACR (2.5 mM), MT (50 μM), or ACR + MT cotreatment for 24 h. DNA condensation in cells was investigated using Hoechst 33258 nuclear staining with an inverted fluorescent microscope (scale bar: 25 μm) (a). Arrows in photos refer to the positive cells. Experimental rats were divided into four groups: control (equal volume normal saline) group, ACR (40 mg⋅kg/day) treatment group, MT (5 mg/kg/day, i.p) treatment group, and ACR + MT simultaneously treatment group. The features of DNA damage were exhibited by a comet assay (scale bar: 50 μm) (b). Tail length, tail DNA%, and olive tail-moment (OTM) were statistically analyzed to assess the degree of DNA damage in rat cerebellum (c) and cerebral cortex (d). The results are expressed as the mean ± SD (). ,   versus the vehicle control group, ,   versus the ACR treatment group.

(a)

(b)

(c)

(d)