Lico A Enhances Nrf2-Mediated Defense Mechanisms against t-BHP-Induced Oxidative Stress and Cell Death via Akt and ERK Activation in RAW 264.7 Cells

Figure 1

Effects of Lico A on t-BHP-induced RAW 264.7 cell cytotoxicity, apoptosis, and necrosis. (a) RAW 264.7 cells were treated by 0, 1, 2.5, 10, 25, 50, and 100 μM t-BHP for 24 h. (b) Cells were pretreated with Lico A (1.85, 3.7 and 7.4 μM) for 18 h, subsequently exposed to t-BHP (10 μM) for 3 h. Cell viability after t-BHP exposure was measured by MTT assay. (c) Cells were exposed to various concentrations of Lico A for 18 h and subsequently subjected to t-BHP (10 μM) for 3 h. The percentage of cell apoptosis and necrosis were determined using flow cytometry. (a and b) represent necrosis and apoptosis, respectively. All results were expressed as means ± SEM of three independent experiments. , versus the control group; ^#, ^## versus the -BHP group.

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