Lico A Enhances Nrf2-Mediated Defense Mechanisms against t-BHP-Induced Oxidative Stress and Cell Death via Akt and ERK Activation in RAW 264.7 Cells

Figure 2

Effects of Lico A on t-BHP-induced ROS generation, GSH levels, and GCLM and GCLC genes expression in RAW 264.7 cells. RAW 264.7 cells were pretreated with or without Lico A for 18 h and then were exposed to t-BHP for additional 3 h. (a) Effect of Lico A on t-BHP-induced ROS generation in RAW 264.7 cells. The ROS generation was determined in accordance with the Experimental Section. (b) Effect of Lico A on t-BHP-induced GSH depletion was determined using a commercial GSH test kit. (c) Effects of Lico A on GCLM and GCLC genes expression. Total RNA was extracted from RAW 264.7 cells and genes expression was quantified using real-time PCR. All results were expressed as means ± SEM of three independent experiments. , versus the control group; ^#, ^## versus the t-BHP group.

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