Lico A Enhances Nrf2-Mediated Defense Mechanisms against t-BHP-Induced Oxidative Stress and Cell Death via Akt and ERK Activation in RAW 264.7 Cells

Figure 7

Effects of Lico A-induced Akt and ERK activation on HO-1 expression and Nrf2 nuclear translocation. Cells were pretreated with LY294002 (10 μM) and U0126 (10 μM) for 6 h and then were exposed to Lico A (3.7 μM) for 18 h. The whole cells lysates were examined by Western blot analysis with anti-HO-1 and anti-β-actin antibodies, and nuclear extracts were subjected to Western blot analysis with anti-Nrf2 and anti-Lamin B antibodies. (b and d) The relative density of protein was performed by densitometric analysis; β-actin and Lamin B acted as an internal control, respectively. All results were expressed as means ± SEM of three independent experiments. versus the control group; ⁺⁺ versus the Lico A group.

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