Downregulation of Endogenous Hydrogen Sulfide Pathway Is Involved in Mitochondrion-Related Endothelial Cell Apoptosis Induced by High Salt

Figure 2

Changes in superoxide anion generation in human umbilical vein endothelial cells (HUVECs). (a) Fluorescent micrographs of superoxide anion generation in HUVEC detected by DHE probes. Red fluorescence indicates levels of superoxide anion generation in HUVEC; (b) quantification analysis of fluorescent intensity of superoxide anion generation in HUVEC detected by CellROX Green Reagent on Fluoroskan Ascent Fluorometer; (c) superoxide anion generation in HUVEC mitochondria detected by MitoSOX probes. Control: the cell treated with 137 mmol/L sodium. HS: high salt, the cell treated with 200 mmol/L sodium for 6 h. HS + NaHS: the cell pretreated with 200 μmol/L NaHS for 30 min following 200 mmol/L sodium for 6 h. versus control group; versus HS group.

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