Downregulation of Endogenous Hydrogen Sulfide Pathway Is Involved in Mitochondrion-Related Endothelial Cell Apoptosis Induced by High Salt

Figure 3

Changes in mitochondrial membrane potential and mitochondrial permeability transition pore (MPTP) opening in human umbilical vein endothelial cells (HUVECs). (a) Change of mitochondrial membrane potential detected by JC-1 fluorescent probe and examined by laser confocal microscope, with red fluorescence presenting JC-1 aggregate and green JC-1 monomer. (b) Changes of MPTP opening in HUVEC detected by calcein-AM as a fluorescence indicator by laser confocal microscopy. The green fluorescence quenching represented MPTP opening. Control: the cell treated with 137 mmol/L sodium. HS: high salt, the cell treated with 200 mmol/L sodium for 6 h. HS + NaHS: the cell pretreated with 200 μmol/L NaHS for 30 min following 200 mmol/L sodium for 6 h.