Genome-Wide Expression Profiling of Anoxia/Reoxygenation in Rat Cardiomyocytes Uncovers the Role of Mito in Energy Homeostasis

Figure 2

Adult rat cardiomyocytes and their status tests after mitoK_ATP opening. (a) Light microscopic morphology of freshly isolated adult cardiomyocytes. They were rod-shaped, with sharp outlines and clear cross striations. (b-c) The effect of DZ and 5HD on the in adult rat cardiomyocytes. At the end point of reoxygenation, cells of Con, A/R, DZ, and DZ5HD group were pretreated with 10 μM Fluo-3-AM and incubated for 60 min at 37°C and measured with a confocal laser microscope. (b) The fluorescence image of cardiomyocytes in four groups. (c) The fluorescence intensity comparison. in A/R group was increased compared with the Con. Applying of DZ reduced the fluorescence intensity. After 5-HD administration, fluorescence intensity increased. (d) Cell viability test with CCK-8 kit. At the end point of reoxygenation, 30 μL CCK-8 was added into M199 to form a 3% CCK-8 resulting solution. Cells were incubated for 1 h before the mixture’s OD value was detected at 450 nm. Cardiomyocytes of A/R group possessed lower level of cell viability when compared with Con. DZ group contained higher level of cell viability when compared with A/R group. Cells in DZ5HD showed the lowest level of cell viability in the 4 groups. Data are mean ± SD. Replication number for each group is marked on the columns. .

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