Cardiac H₂S Generation Is Reduced in Ageing Diabetic Mice

Figure 5

Fifteen months of high-fructose feeding alters CBS, CSE, and 3-MST protein expression. (a) Representative Western blots and quantitative analysis for CBS, CSE, and 3-MST expression in the myocardium. GAPDH was used as the internal control. (b) Representative double-staining immunofluorescence showing the distribution of CBS (red) in the cardiomyocytes and vessels (labelled by CD31, green) from control or ageing diabetic mice (scale bar = 250 μm). Results are means ± SEM. A of <0.05 was considered significant.

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