Kinetic Modeling Reveals the Roles of Reactive Oxygen Species Scavenging and DNA Repair Processes in Shaping the Dose-Response Curve of KBrO₃-Induced DNA Damage

Figure 1

Base model. Our model consists of a series of reactions based on a mechanism proposed by Kawanishi and Murata [17]. Bromate () reacts with GSH to form a reactive intermediate complex. (BrOI1). This reactive intermediate receives an electron from Guanine, leading to base oxidation and formation of an 8-OH-dG adduct. This damage cycle can repeat twice more with and , giving each bromate molecule three opportunities to form an adduct. Adduct repair is handled by a base excision repair (BER) mechanism that has a small chance of repair failure resulting in single strand breaks (SSB). A scavenger of the reactive intermediates and additional SSB repair mechanisms (break repair) were added in specific cases. Round-end arrows indicate enzymatic participation in a reaction.