Research Article
Neuroprotective Effect of Brassica oleracea Sprouts Crude Juice in a Cellular Model of Alzheimer’s Disease
Figure 2
Protective effect of broccoli sprouts juices on Aβ25–35-induced apoptosis. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 24, 48, and 72 h. (a) Morphological analysis of apoptosis was achieved by staining of cell nuclei with Hoechst 33258. Arrows indicate the apoptotic cells. Images are representative of three different experiments. (b) Quantification of the percentage of apoptotic cells by fluorescence microscopy with the DNA intercalating Hoechst 33258. Data are expressed as mean ± SEM (), and versus control; , , and versus Aβ25–35; versus juice A. (c) Analysis by flow cytometry of the sub-G1 peak in cells stained with propidium iodide (PI). The percentage of cells with hypodiploid DNA content is shown as function of the PI fluorescence signal (FL2-A). Histograms are representative of three independent experiments.
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