Research Article
Neuroprotective Effect of Brassica oleracea Sprouts Crude Juice in a Cellular Model of Alzheimer’s Disease
Figure 4
Broccoli sprouts juices protect SH-SY5Y cells from Aβ25–35-mediated apoptosis through upregulation of HSP70 gene expression. (a) SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 1, 4, 7, 24, 48, and 72 h. After treatment total RNA was extracted and used for qRT-PCR determination of HSP70 gene induction. The RSP27A housekeeping gene was used as a control for normalization of the data. The relative amounts of gene expression were calculated by the comparative threshold cycle (ΔΔCT) method. The intracellular levels of mRNA are expressed as fold induction compared to control cells at 0 h of incubation. (b) Cell lysates obtained after 7 h of treatment were subjected to western blot analysis with anti-Hsp70 antidoby. Actin levels were compared to ensure equal amount of protein loading. The intracellular Hsp70 protein levels in the samples were expressed as a percentage compared to the control. Data are represented as mean ± SEM (), and versus control; and versus Aβ25–35.
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