Neuroprotective Effect of Brassica oleracea Sprouts Crude Juice in a Cellular Model of Alzheimer’s Disease

Figure 5

Protective effect of broccoli sprouts juices from Aβ_25–35-induced GSH depletion. SH-SY5Y cells were incubated with 25 μM Aβ_25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 1, 4, 7, 24, 48, and 72 h. After treatment, total cell lysate was prepared for the determination of intracellular GSH levels by RP-HPLC analysis, using the fluorescence signal of the derivatized product with OPA. The concentration of GSH in the samples, normalized by the number of cells, was expressed as a percentage compared to the control. Data are represented as mean ± SEM () , , and versus control; and versus Aβ_25–35; versus (A + Aβ).