Research Article
Neuroprotective Effect of Brassica oleracea Sprouts Crude Juice in a Cellular Model of Alzheimer’s Disease
Figure 6
Broccoli sprouts juices upregulate the cellular antioxidant defence capacity. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 1, 4, 7, 24, 48, and 72 h. After treatment, total RNA was extracted and used for qRT-PCR determination of gene expression of (a) HO-1; (b) TRX; (c) TrxR. The RSP27A housekeeping gene was used as a control for normalization of the data. The relative amounts of gene expression were calculated by the comparative threshold cycle (ΔΔCT) method. The intracellular levels of mRNA are expressed as fold induction compared to control cells at 0 h of incubation. (d) Total cell lysates were subjected to spectrophotometric assay of NQO1 activity which was expressed as a percentage compared to the control. Data are represented as mean ± SEM (), , , and versus control; , , and versus Aβ25–35; , , and versus juice A; , , and versus (A + Aβ).
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