Research Article
Neuroprotective Effect of Brassica oleracea Sprouts Crude Juice in a Cellular Model of Alzheimer’s Disease
Figure 7
Broccoli sprouts juices activate nuclear translocation of Nrf2 transcription factor which controls the expression of cell survival genes involved in defence against oxidative stress-mediated damage. SH-SY5Y cells were incubated with 25 μM Aβ25–35 in the presence or in the absence of 10 μL/mL of juice A or juice B for 3 h. (a) Nrf2 nuclear translocation was verified by immunofluorescence microscopy by utilizing anti-Nrf2 antibody and DAPI staining. Representative images of three independent experiments show the stimulation of Nrf2 nuclear accumulation by broccoli sprouts juices A or B exposure, compared to both control cells and Aβ25–35 alone treated cells. (b) Densitometric analysis of the mean nuclear located-Nrf2 fluorescence signal as calculated analyzing immunofluorescence images by the Image J software. Data are expressed as percentage of control and represented as mean ± SEM (), versus control; versus Aβ25–35; versus juice A; versus (A + Aβ).
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