GC-Rich Extracellular DNA Induces Oxidative Stress, Double-Strand DNA Breaks, and DNA Damage Response in Human Adipose-Derived Mesenchymal Stem Cells

Figure 4

Changes in the amount of proliferation markers Ki-67 and PCNA in haMSCs exposed to gDNA or GC-DNA at final concentration 50 ng/mL (FACS). (a) (A) fixed cells stained with anti-Ki-67 (FITC) antibodies. Distribution of fluorescence intensities of the cells exposed to DNAs. Background fluorescence was quantified using FITC-conjugated secondary antibodies (grey color); (B) the average of the median signal intensity of FL1 (Ki-67) in haMSC. (b) (A) fixed cells stained with anti-PCNA (FITC) antibodies. Distribution of fluorescence intensities of haMSCs exposed to DNAs. Background fluorescence was quantified using FITC-conjugated secondary antibodies (grey color). (B) The average of the median signal intensity of FL1 (PCNA). (c) The ratio of the levels of mRNA CCND1, CDKN2, and CDKN1A in cells exposed to gDNA, pBR322, or GC-DNA. The incubation time is indicated on the histogram. Data points were averaged and represented as mean ± SD for three biological replicates. against control, nonparametric U test.

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