Dietary Tocotrienol/γ-Cyclodextrin Complex Increases Mitochondrial Membrane Potential and ATP Concentrations in the Brains of Aged Mice

Table 2

Primer sequences and annealing temperatures used for qRT-PCR analyses in murine brain tissue.

Gene symbol	Gene name	Forward 5′-3′	Reverse 3′-5′	Annealing temperature
Actb	Actin, beta	GACAGGATGCAGAAGAGATTACT	TGATCCACATCTGCTGGAAGGT	55°C
Cat	Catalase	GGAGCAGGTGCTTTTGGATA	CTGACTCTCCAGCGACTGTG	55°C
Gclm	Glutamate-cysteine ligase, modifier subunit	TCCCATGCAGTGGAGAAGAT	AGCTGTGCAACTCCAAGGAC	57°C
Gpx4	Glutathione peroxidase 4	ATGAAAGTCCAGCCCAAGG	CGGCAGGTCCTTCTCTATCA	59°C
Hmox1	Heme oxygenase 1	GAGCCTGAATCGAGCAGAAC	AGCCTTCTCTGGACACCTGA	59°C
Sod2	Superoxide dismutase 2, mitochondrial	GCCTGCTCTAATCAGGACCC	TAGTAAGCGTGCTCCCACAC	59°C