Hydrogen Sulfide Alleviates Cadmium-Induced Cell Death through Restraining ROS Accumulation in Roots of Brassica rapa L. ssp. pekinensis

Figure 3

Effect of H₂S on Cd-induced root cell death in Chinese cabbage. (a) Roots stained with Evans blue observed under a light microscope and quantitative analysis of cell death caused by Cd stress. (b) DNA damage analysis. (c) Root growth phenotypes. CK: control; NaHS: fumigated with 5 M NaHS for 24 h; Cd: 5 mM Cd treatment for 48 h; NaHS + Cd: seedlings fumigated with 5 M NaHS for 24 h and then treated with 5 mM Cd for 48 h; HA + Cd: seedlings treated with 1 mM HA for 4 h and then treated with 5 mM Cd for 48 h; HA: seedlings treated with 1 mM HA for 4 h. Data are mean ± SE of three independent repeats. LSD was used for multiple comparisons; different letters indicate significant differences ().

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