A Nitric Oxide-Donor Furoxan Moiety Improves the Efficacy of Edaravone against Early Renal Dysfunction and Injury Evoked by Ischemia/Reperfusion

Figure 5

Effects of EDV and NO-EDV on Akt and eNOS phosphorylation. Representative Western blot and corresponding densitometric analysis of the bands showing phosphorylated (Ser⁴⁷³) and total Akt (panel (a)) and phosphorylated (Ser¹¹⁷⁷) and total eNOS in rats exposed to I/R in the absence (vehicle) or presence of EDV (6 and 30 μmol/kg, i.v.) or NO-EDV (1.2 and 6 μmol/kg, i.v.). Each immunoblot is from a single experiment and is representative of three separate experiments. Densitometric analysis of the related bands is expressed as relative OD, corrected for the corresponding β-actin contents, and normalized using the related sham-operated band. The data from bands densitometric analysis are mean S.E.M. versus vehicle.

(a)

(b)