Enhanced Locomotor Activity Is Required to Exert Dietary Restriction-Dependent Increase of Stress Resistance in Drosophila

Figure 1

Wing clipping attenuates an increase of locomotor activity induced by dietary restriction in Drosophila. (a) In each experimental group, normal or wing-clipped flies were reared on either ad libitum (AL, 5% YE) or restricted (DR, 0.5% YE) diet. At the age of 10 days, flies’ spontaneous locomotor activity was measured from each group with 10 min binning, which was plotted as a function of time. Each solid and dotted line represents mean locomotor activity calculated from 8 independent experiments. The white and black bars indicate the light and dark cycle where flies are housed. (b) The data presented in (a) was replotted as a bar graph to demonstrate flies’ total spontaneous locomotor activity for 24 hrs. Data were presented as mean ± S.E.M. ; N.S. stands for nonsignificant. One-way ANOVA with Bonferroni post hoc test.

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