Coenzyme Q10 Inhibits the Aging of Mesenchymal Stem Cells Induced by D-Galactose through Akt/mTOR Signaling

Figure 3

Effects of different concentrations CoQ10 on ROS generation in MSCs. (a) DCFH staining. In the 1, 10, and 100 μmol/L CoQ10 group, little DCFH-stained cells were observed under a fluorescence microscope compared with that in the D-gal control group. Green: DCFH staining; blue: Hoechst 33342 staining. Scale bar = 25 μm. (b) Quantification of intracellular ROS level. The DCFH fluorescence intensity in the 1, 10, and 100 μmol/L CoQ10 group was evidently lower compared with the D-gal control group (, ; ). (c) MitoSOX staining. In the 1, 10, and 100 μmol/L CoQ10 group, MitoSOX fluorescence brightness was clearly lower than that in the D-gal control group. Red: MitoSOX staining; blue: Hoechst 33342 staining. Scale bar = 25 μm. (d) Quantification of mitochondrial ROS level. The MitoSOX fluorescence intensity in the 1, 10, and 100 μmol/L CoQ10 group was significantly lower compared with the control group (; ).

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