Ginkgolide B Inhibits JAM-A, Cx43, and VE-Cadherin Expression and Reduces Monocyte Transmigration in Oxidized LDL-Stimulated Human Umbilical Vein Endothelial Cells

Figure 5

Ginkgolide B and LY294002 inhibit Akt phosphorylation and LY294002 suppresses JAM-A, Cx43, and VE-cadherin expression in ox-LDL-treated HUVECs. (a) The upper panel showed that ginkgolide B inhibits Akt phosphorylation. The lower panel is density analysis of Akt phosphorylation. (b) The upper panel showed that LY294002 inhibits Akt phosphorylation. The lower panel is density analysis of Akt phosphorylation. (c) The upper panel showed LY294002 diminishes JAM-A expression. The lower panel is density analysis of JAM-A expression. (d) The upper panel showed that LY294002 diminishes Cx43 expression. The lower panel is density analysis of Cx43 expression. (e) The upper panel showed LY294002 decreases VE-cadherin expression. The lower panel is density analysis of VE-cadherin expression. The data were obtained from three independent experiments. , ox-LDL-treated cells versus non-ox-LDL-treated cells; , ox-LDL-treated cells versus ginkgolide B-treated cells.

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