Oxidative Medicine and Cellular Longevity / 2015 / Article / Fig 1

Research Article

Increased Susceptibility of Gracilinanus microtarsus Liver Mitochondria to Ca2+-Induced Permeability Transition Is Associated with a More Oxidized State of NAD(P)

Figure 1

Oxidative phosphorylation parameters in mouse and marsupial liver mitochondria. (a) Mus musculus (mouse) and Gracilinanus microtarsus (marsupial) isolated liver mitochondria (0.5 mg/mL) were incubated in standard reaction medium containing NADH-linked respiratory substrates (2 mM malate, 1 mM pyruvate, 1 mM α-ketoglutarate, and 1 mM glutamate) and 200 μM EGTA. Respiratory states were determined under basal conditions (V2) and after sequential additions of ADP (300 μM) and 1 μg/mL oligomycin (Oligo) to achieve respiratory states 3 (V3) and 4 (V4), respectively. The results are expressed as means ± SEM (). versus mouse mitochondria. (b) Respiratory control ratios (V3/V4). (c) Representative traces of oxygen consumption by mouse and marsupial liver mitochondria (LM).

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