Oxidative Medicine and Cellular Longevity / 2015 / Article / Fig 3

Research Article

Flaxseed Oil Containing α-Linolenic Acid Ester of Plant Sterol Improved Atherosclerosis in ApoE Deficient Mice

Figure 3

Effects of FO+ALA-PS on mRNA and protein expressions of hepatic HMGCR (a-b), SREBP-2 (c-d), PPARα (e-f), and SREBP-1c (g-h) in mice. After intervention by flaxseed oil with ALA-PS for 18 weeks, total RNA was extracted from liver of mice by Trizol. HMGCR, SREBP-2 PPARα, and SREBP-1c mRNA expressions were analyzed by real-time RT-PCR. The mRNA of β-actin was quantified as an endogenous control. Hepatic lysates were prepared and immunoblotted with corresponding antibody, respectively. Blotting with anti-β-actin was used as a protein loading control. HMGCR, SREBP-2, PPARα, and SREBP-1c are presented as fold change relative to control. Representative immunoblots are shown. Values are given as mean ± standard deviation of the mean (). versus the control; versus the HFD group; versus the FO group.
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