Flaxseed Oil Containing α-Linolenic Acid Ester of Plant Sterol Improved Atherosclerosis in ApoE Deficient Mice

Figure 6

Effects of FO+ALA-PS on mRNA and protein expressions of MCP-1 (a-b), IL-1β (c-d), and IL-6 (e-f) in circulating monocytes of mice. After supplementing flaxseed oil with ALA-PS for 18 weeks, total RNA was extracted from circulating monocytes of mice by Trizol. MCP-1, IL-1β, and IL-6 mRNA expressions were analyzed by real-time RT-PCR. The mRNA of β-actin was quantified as an endogenous control. Monocytes lysates were prepared and immunoblotted with corresponding antibody, respectively. Blotting with anti-β-actin was used as a protein loading control. MCP-1, IL-1β, and IL-6 are presented as fold change relative to control. Representative immunoblots are shown. Values are given as mean ± standard deviation of the mean (). versus the control; versus the HFD group; versus the FO group.

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