Flaxseed Oil Containing α-Linolenic Acid Ester of Plant Sterol Improved Atherosclerosis in ApoE Deficient Mice
Effects of FO+ALA-PS on mRNA and protein expressions of (a-b), (c-d), (e-f), and (g-h) in aorta of mice. Total RNA was extracted from aortas of mice by Trizol. , , , and mRNA expressions were analyzed by real-time RT-PCR. The mRNA of β-actin was quantified as an endogenous control. Aortic lysates were prepared and immunoblotted with corresponding antibody, respectively. Blotting with anti-β-actin was used as a protein loading control. p22phox, p47phox, p67phox, and gp91phox are presented as fold change relative to control. Values are given as mean ± standard deviation of the mean (). versus the control; versus the HFD group; versus the FO group.
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