Oxidative Stress during the Progression of β-Amyloid Pathology in the Neocortex of the Tg2576 Mouse Model of Alzheimer’s Disease

Figure 7

SOD1 and SOD2 protein levels and distribution in WT and Tg neocortex. (a) Densitometric values of SOD1 WB of protein extracts from 3-, 6-, 9-, 12-, and 18-month-old WT and Tg neocortex. Data are expressed as mean ± SD. ; . (b) SOD1 IHC in WT and Tg neocortex. Higher expression levels in Tg than in WT neurons are found at 12 months, while the opposite is true for 18 months. Scale bars, 40 μm. (c) Densitometric values of SOD2 WB of protein extracts from 3-, 6-, 9-, 12-, and 18-month-old WT and Tg neocortex. Data are expressed as mean ± SD. ; ; . (d) SOD2 IHC in WT and Tg neocortex. Relatively low immunoreactivity levels in Tg neurons are found at 3 and 18 months, while at 12 months SOD2 expression is induced in Tg. Note striking immunostaining in glial cells surrounding and infiltrating amyloid plaques. Scale bars, 40 μm.