Hydrogen Sulfide Prevents Advanced Glycation End-Products Induced Activation of the Epithelial Sodium Channel

Figure 3

H₂S ameliorates AGE- or 3-AT-elicited oxidative stress and AGEs reduce catalase expression in A6 cells. (a) The left image shows that there was a residual level of intracellular ROS under control condition; the middle image shows a significant increase in intracellular ROS upon application of basolateral 200 μg/mL AGEs; the right image shows that the AGE-induced increase in intracellular ROS was abolished by 0.1 mM NaHS treatment. (b) The left image shows that there was a residual level of intracellular ROS under control conditions; the middle image shows a significant increase in intracellular ROS upon application of apical 20 mM 3-AT; the right image shows that the 3-AT-induced increase in intracellular ROS was also abolished by 0.1 mM NaHS treatment. (c) and (d) Summarized bar graphs show the mean fluorescence intensities under indicated experimental conditions ( for each experimental condition; indicates compared to control). (e) and (f) Western blot demonstrating that expression levels of catalase were suppressed by AGEs (, represents compared to control).

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