Hydrogen Sulfide Prevents Advanced Glycation End-Products Induced Activation of the Epithelial Sodium Channel

Figure 5

AGEs activate ENaC via PI3K and PTEN signaling pathways. (a) and (b) ENaC activity in A6 cells treated either with basolateral 200 μg/mL BSA or with basolateral 200 μg/mL AGEs, before and after addition of 30 nM to the apical bath. (c) and (d) ENaC activity in A6 cells treated as in (a) and (b), before and after addition of 5 μM LY294002 to the apical bath; the data show that a PI3K inhibitor, LY294002, significantly inhibits ENaC activities under control condition and in the presence of AGEs. Four breaks between the traces indicate 20 min omitted recording periods. Summarized of ENaC before and after application of each reagent were shown on the right. paired experiments. indicates .

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