Tanshinol Rescues the Impaired Bone Formation Elicited by Glucocorticoid Involved in KLF15 Pathway

Figure 7

Regulation of differential genes by KLF15 and involvement of Osterix in protective effect of tanshinol on bone formation. (a) MC3T3-E1 cells were infected with recombinant adenovirus Ad-KLF15 for 4 days. mRNA expression of bone formation-related genes and KLF15 gene was measured by qRT-PCR. (b) MC3T3-E1 cells transfected with KLF15 siRNA for 18 h. mRNA expression of bone formation-related genes and KLF15 gene was measured by qRT-PCR. (c) MC3T3-E1 cells were infected with Osterix-luc reporter plasmid in combination with recombinant adenovirus Ad-KLF15 or mock (noninfection). (d) MC3T3-E1 cells were cotransfected with the Osterix-luc reporter plasmid in combination with KLF15 siRNA or the scrambled sequence. Luciferase activity assays were explored using the Dual-Luciferase Reporter Assay System as described under Section 2.8 in Materials and Methods. The data represent mean ± SD of luciferase relative luminescence units (RLU) normalized to corresponding renilla luciferase activity (triplicates). versus indicated group in cells exposed to empty vector (mock) or scrambled control; versus indicated group in cells exposed to KLF15 siRNA or recombinant adenovirus Ad-KLF15; versus corresponding mock; versus corresponding scrambled control.

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