Partial Oxygen Pressure Affects the Expression of Prognostic Biomarkers HIF-1 Alpha, Ki67, and CK20 in the Microenvironment of Colorectal Cancer Tissue

Figure 4

Immunohistochemical analysis for the expression levels of CK20 in different-size CRC tissues. The numbers 1, 2, 3, 4, and 5 represent small-size CRC tissues (<300 mm³, ), middle-size CRC tissues (301–600 mm³, ), big-size CRC tissues (601–900 mm³, ), and super-size CRC tissues (>900 mm³, ) under 200x magnification and the gray values of CK20, respectively. The letters (A), (B), and (C) represent Finefix, formalin-fixed, and Finefix-plus-microwave-fixed tissues, respectively. via a small-size CRC tissue. Scale bar: 50 μm. The bars in the boxes were average activities and the boxes represented 90% of the samples. The error bars were above or below the boxes.